Fig. 6

Overexpression of SNAIL is not sufficient to initiate EMT in absence of ZEB1 and FOXC2. (a) Integrating FOXC2 to the TCS framework – TCS-FOXC2. (b) Top panel: Bifurcation diagram of the TCS-FOXC2 circuit when FOXC2 is present, as reflected by S _I  = 0. Bottom panel: Bifurcation diagram of the TCS-FOXC2 circuit when FOXC2 is absent, as reflected by S _I  = 2^∗10⁵ molecules. (c) Western blot analysis of EMT markers upon SNAIL overexpression in HMLE cells, and FOXC2 suppression in HMLE–Snail cells respectively. (d) The proposed EMT regulatory framework integrating transcriptional regulation by FOXC2