Fig. 5
From: Probing three-dimensional collective cancer invasion with DIGME
A two-layer DIGME device. A1: Schematics of the two-layer device. A circular MDA-MB-231 diskoid is confined by 1.5 mg/ml collagen matrix (ECM 1). ECM 1 is inside of 3 mg/ml collagen matrix (ECM 2). A2: 3D, top, and side views of the device. Green: MDA-MB-231 cells. Red: ECM 1 labeled with red fluorescent particles. Blue: ECM 2 labeled with far-red fluorescent particles. A3: confocal reflection image showing the collagen fibers at the interface of ECM 1 and ECM 2. B-E Top views of the invading diskoid at day 0, 5, 10 and 15. F: Invasion distance d f as a function of time