Fig. 1
From: Probing three-dimensional collective cancer invasion with DIGME
Preparation and collective migration of a circular DIGME. A Schematics showing the steps of forming a DIGME device. B1-B3 Top views of a diskoid in 3D ECM. GFP-labeled MDA-MB-231 cells are cultured in DIGME device and confocal imaging are performed at day 1, 5 and 10. Bottom insets show the corresponding side views. C1-C3 Manually identified 3D cell centers and morphological phenotypes corresponding to B1-B3. Green: rounded cells with aspect ratio less than 2. Red: elongated cells with aspect ratio greater than 2